The Queen's Blood was part of Lady Dahlia's collection, until it was stolen by Don Paolo several weeks prior London Life. He planned to use it to create a weapon of destruction. Lady Dahlia tried to get the Family to help her get it back, but instead learned of the 'curse' behind it. She believed Flora's life was in danger. The curse, however, was just many rumors about fairies and kidnapping that Don Paolo had spread; he planned to take over the underground ruins while their attentions were focused on that.
Layton and the player meet up again at Crumpet Hotel to make up a plan to save Little London. The player is sent to Golden Spires Casino with a message for Lady Dahlia and Flora, before being entrusted with the Sewer Key and the Queen's Blood and sent down to the ruins. The Queen's Blood shakes the ground and makes a big red glow.